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. 2015 Feb 2;290(13):8067–8080. doi: 10.1074/jbc.M114.633081

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — MiR-3189-3p is up-regulated and incorporated into the RNA-induced silencing complex in cells treated with fenofibrate. A, Western blot to detect Ago2 after immunoprecipitation of lysates obtained from untreated (Untr) and fenofibrate-treated cells. Left over IP represents the fraction of lysates obtained after overnight incubation with Ago2 antibody or the control isotype IgG and is used as a negative control. B, real-time PCR to detect miR-3189-3p. Results are expressed as -fold change of the microRNAs in fenofibrate-treated cells compared with untreated cells. The enrichment of microRNAs or mRNAs in the RNA-induced silencing complex was calculated according to the formula 2^{−(CtAgo2 − CtIgG)} and normalized over RNU6B. ctrl, control. C and D, relative expression of GDF15 and miR-3189-3p under the indicated conditions (treatments and transfections) compared with controls. *, p < 0.05 compared with the respective controls.