FIGURE 1.

Cyr61 mediated PDGF-induced SMC migration but not proliferation. A, PDGF-BB induced Cyr61 protein expression in mouse aortic SMCs in a time-dependent manner. Cultured cells were starved for 24 h prior to PDGF-BB stimulation for various times as indicated. Cell lysates were subjected to Western blot analysis. β-actin served as the loading control. Top panel, representative Western blot result from three independent experiments. Bottom panel, results of the Western blot analysis quantified by densitometry. *, p < 0.05; **, p < 0.01 versus control. B, PDGF-BB dose-dependently induced Cyr61 protein expression in SMCs. Top panel, representative Western blot result from three independent experiments. Bottom panel, results of Western blot analysis quantified by densitometry. **, p < 0.01 versus control. C, Western blot results of knockdown of Cyr61 expression. Non-silencing siRNA (Non) served as a control. D, PDGF-BB dose-dependently induced SMC migration, and knockdown of Cyr61 expression with the specific Cyr61 siRNA largely blocked PDGF-BB-induced SMC migration. *, p < 0.05; **, p < 0.01 versus control; ##, p < 0.01 versus non-silencing siRNA group. E, knockdown of Cyr61 expression with specific siRNA had no effect on PDGF-BB-induced SMC proliferation. *p < 0.05, **p < 0.01 versus controls. F, Western blot results of the effects of knockdown of Cyr61 expression on the expression or phosphorylation of PDGF receptor β. G, Western blot result of Cyr61 overexpression after cells were infected with Ad-CMV-mCyr61 adenovirus compared with control cells infected with Ad-CMV-null adenovirus. β-actin served as the loading control. IB, immunoblot. H, effect of overexpression of Cyr61 on SMC migration (transwell migration assay in serum-free medium). **, p < 0.01 versus control. I, effect of overexpression of Cyr61 on SMC proliferation.