FIGURE 3.

PDGF-BB-induced Cyr61 protein was secreted into the ECM. A, Western blot analysis of SMCs detached from culture dishes. Cells were detached from the dishes with 1 mm EDTA at various time points as indicated and lysed in the lysis buffer, followed by Western blot analysis. β-actin was used to assess protein loading. B, Western blot analysis of ECM proteins. ECM remaining on the culture dishes was extracted in Laemmli sample buffer (described under “Experimental Procedures”) and then subjected to Western blot analysis.