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. 2015 Feb 6;290(13):8294–8309. doi: 10.1074/jbc.M114.591800

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Rv3134c-devR-devS operon is activated during aerobic nitrate metabolism. A, expression of Rv3134c-devR-devS genes was determined in M. tuberculosis H37Rv aerobic cultures grown in 7H9T medium with or without nitrate by qRT-PCR. M. tuberculosis H37Rv grown in the absence of nitrate served as a control. B, induction of Rv3134c-devR-devS operon under nitrate metabolism conditions is phosphorylation-dependent. The transcript levels of devR and two well established DevR-regulated genes, narK2 and hspX, were analyzed by qRT-PCR in M. tuberculosis H37Rv, ΔdevR, and ΔdevSΔdosT cultures cultivated in the presence of nitrate. The expression was normalized using 16 S rRNA gene, and the -fold change in expression of all genes was calculated as described for Fig. 3. ** and ***, p < 0.01 and p < 0.001, respectively. Error bars, S.D.