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. 2015 Feb 6;290(13):8294–8309. doi: 10.1074/jbc.M114.591800

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Co-regulation is independent of NarL phosphorylation but requires DevR activation. A, role of NarL phosphorylation in cooperative binding of NarL and DevR on the acg (Rv2032) promoter region. NarL or NarLD61N (1.5 μg) and DevR (28 ng) proteins were used in the EMSAs as depicted. B, role of NarL phosphorylation in cooperative binding of NarL and DevR on the narK2 promoter. All lanes contain 2.0 pmol of narK2 probe. NarL or NarLD61N (9 μg) and DevR (40 ng) proteins were used in the EMSAs as depicted.