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. 2015 Feb 5;290(13):8331–8347. doi: 10.1074/jbc.M114.615088

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — mRNA decapping factors exert partially redundant functions in rapid mRNA decay from the 5′ end by tethered TNRC6A. A, interaction of FLAG-MS2-Mid with yeast Dhh1-HA. Cell lysates of wild type cells transformed with the indicated plasmids were immunoprecipitated using anti-FLAG antibody. Total extract (Input) and immunoprecipitate (IP) were analyzed by Western blotting using anti-HA or anti-FLAG antibody. B, interaction of FLAG-Dhh1 with Not1-HA. Immunoprecipitation was performed as described in A. C, mRNA fate modulators Dhh1 and Pat1 have partially redundant functions in TNRC6AMid-mediated 5′-to-3′ mRNA decay independent of translation and a poly(A) tail. Top, Northern blotting analysis of No-AUG-MS2-Rz mRNA in the indicated cells. The half-lives of mRNA in the indicated cells are shown as the mean values of three independent experiments, with S.D. values. Bottom, graphs of the half-life analysis. The p value was calculated by using Student's t test.