FIGURE 7.

The roles of decapping factors in TNRC6A-mediated translation repression in yeast. A–E, cell extracts were prepared from wild type and the indicated mutant cells transformed with the indicated plasmids, and polysome analysis was performed. Distributions of the reporter RNAs were analyzed by Northern blotting. F, pulse-labeling experiments were performed using dhh1Δ, pat1Δ, and dhh1Δpat1Δ mutant cells. GFP-MS2 and GFP-MS2-Rz protein levels reflect the mean values of three independent experiments, with S.D. values.