TABLE 1.

Yeast strains and plasmids used in this study

Strain/Plasmids	Genotype/plasmid	Source
Strains
W303-1a	MATa ade2 his3 leu2 trp1 ura3 can1	Laboratory stock
YIT2007	W303-1a xrn1Δ::kanMX	Ref. 49
YIT2013	W303-1a ski2Δ::kanMX	Ref. 67
YIT2030	W303-1a pat1Δ::natMX	This study
YIT2031	W303-1a dhh1Δ::kanMX	This study
YIT2032	W303-1a pat1Δ::natMX4, dhh1Δ::kanMX4	This study
YIT2033	W303-1a ccr4Δ::kanMX	This study
YIT2034	W303-1a caf1Δ::kanMX	This study
Plasmids
pIT2068	pGAL1p-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR) URA3 CEN	Ref. 49
pIT2069	pGAL1p-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR)-MS2 URA3 CEN	Ref. 49
pIT2070	pGAL1p-No-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR) URA3 CEN	Ref. 49
pIT2071	pGAL1p-No-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR)-MS2 URA3 CEN	Ref. 49
pIT2082	pGAL1p-No-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR)-Rz URA3 CEN	Ref. 49
pIT2083	pGAL1p-No-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR)-MS2-Rz URA3 CEN	Ref. 49
pIT2139	pGAL1p-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR)-MS2-Rz URA3 CEN	This study
pIT2140	pGAL1p-AUG-FLAG-MPT4ΔN-PGK1 (3′UTR)-Rz URA3 CEN	This study
pIT2141	pTEF1p-FLAG-MS2 LEU2 CEN	This study
pIT2142	pTEF1p-FLAG-MS2-zTNRC6AMid LEU2 CEN	This study
pIT2143	pGPD1p-Dhh1-HA URA3 CEN	This study
pIT2144	pGPD1p-Pat1-HA URA3 CEN	This study
pIT2145	pGPD1p-Not1-HA URA3 CEN	This study
pIT2146	pTEF1p-FLAG-MS2-dmGW182Mid LEU2 CEN	This study
pIT2147	pGPD1p-Dhh1-FLAG LEU2 CEN	This study
pIT2148	pGPD1p-Dhh1 LEU2 CEN	This study
pIT2149	pGAL1p-GFP-MS2 URA3 CEN	This study
pIT2150	pGAL1p-GFP-MS2-Rz URA3 CEN	This study
pIT2151	pGAL11p-GFP URA3 CEN	This study
pIT2152	pGAL1p-GFP-Rz URA3 CEN	This study
pIT2155	pTEF1p-FLAG-MS2-zTNRC6AMid mutants QSR and W LEU2 CEN	This study
M44	pCS2 + HA-λN-zTNRC6A-Mid-globin3′UTR	Ref. 40
M277	pCS2 + HA-λN-MS2-globin 3′UTR	This study
M338	pCS2 + MT-CNOT7-D40A,E42E,C67E,L71E-sv40	This study
M342	pCS2 + MT-DCP2-E147A,E148A-sv40	This study