Skip to main content
. 2015 Feb 5;290(13):8331–8347. doi: 10.1074/jbc.M114.615088

TABLE 2.

The oligonucleotides used for poly(A) tail analysis and qRT-PCR

Name/Description Sequence
PAT reverse 5′-GGTAATACGACTCACTATAGCGAGACCCCCCCCCCTT-3′
eif4ebp2 PAT forward 5′-CAAACTATCAGAAATGCATTTAGTTCTG-3′
gstm PAT forward 5′-TCAGGTGAGTGTTGCATTTTG-3′
acadl PAT forward 5′-ATCATATGGTCTACGCCGAGAG-3′
gfp forward 5′-AAGGGCATCGACTTCAAGGAG-3′
gfp reverse 5′-GATGCCGTTCTTCTGCTTGTC-3′
fluc qPCR forward 5′-AGAACTGCCTGCGTGAGATTC-3′
fluc qPCR reverse 5′-ACCGTGATGGAATGGAACAAC-3′
act1b qPCR forward 5′-TTGTTGGACGACCCAGACATC-3′
act1b qPCR reverse 5′-ATGGGGTATTTGAGGGTCAGG-3′
eif4ebp2 qPCR forward 5′-ACGACTCAACGCAGCTACCTC-3′
eif4ebp2 qPCR reverse 5′-CGGTCCAACAGGAACTTACGA-3′
gstm qPCR forward 5′-GCTCCTGCACCTCATTTTGAAG-3′
gstm qPCR reverse 5′-ACCGGTGTATTCCAACAGCAG-3′
acadl qPCR forward 5′-CTATGTGATGCAGCGGAAGG-3′
acadl qPCR reverse 5′-TGAATGCCCGACCTACACAG-3′