FIGURE 2.

A, representative Coomassie G-250-stained gel lane of myelin protein separated with SDS-PAGE after photolabeling with 4 μm [³H]AziPm ± 3, 30, or 100 μm propofol. Each gel lane was cut horizontally into 1-mm pieces, and the radioactivity content in each slice is shown in the aligned graph. B, shown are the levels of covalent [³H]AziPm binding to human SIRT2 in solution. In this experiment, SIRT2 was photolabeled with 4 μm [³H]AziPm ± the indicated concentrations of propofol, and no additional substrates were added. Each mean was derived from 3–6 values from separate photolabeling experiments. Mean values are shown with S.E.