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SIRT2 deacetylase assay Western blot and quantification, as in Fig. 5C, but with the microtubule-stabilizing agent epothilone D (epo-D) added to some reactions. Epothilone D did not affect SIRT2 deacetylase activity or propofol inhibition of SIRT2. hSIRT2, human SIRT2; nicot., nicotinamide; Acet-tubulin, acetylated α-tubulin.
