FIGURE 7.

A, SIRT2 deacetylase activity in the presence of propofol, shown as the percentage of control, using the SIRTainty class III histone deacetylase assay kit (EMD Millipore) and the included acetyl peptide substrate. Experimental conditions are described under “Experimental Procedures.” B, SIRT2 activity in the presence of propofol using the SIRT2 inhibitor screening assay (Sigma-Aldrich) and the included fluorophore-conjugated acetyl peptide substrate. Experiments were performed according to the manufacturer's instructions. C, SIRT2 activity using the same kit as in B, with the fluorophore-conjugated acetyl peptide substrate, but with varying concentrations of NAD⁺. For assays in A–C, mean values are shown with S.D.