TABLE 1.
SIRT2 peptides identified with LC-MS/MS as photolabeled by AziPm
| Photolabeled peptidea | Peptide charge | Observed MH+ | Calculated MH+ | Enzyme usedb |
|---|---|---|---|---|
| 131F.FALAKELY#PGQF.K144 | +2 | 1599.8101 | 1599.8069 | Chymo |
| 132F.ALAKELY#PGQF.K144 | +2 | 1452.7406 | 1452.7385 | Chymo |
| 134L.AKELY#PGQF.K144 | +2 | 1268.6200 | 1268.6173 | Chymo |
| 136K.ELY#PGQFK.P145 | +1 | 1197.5819 | 1197.5802 | Trypsin |
| 172L.ERVAGLEPQDLVEAHGTF#Y.T192 | +3 | 2347.1260 | 2347.1216 | Chymo |
| 202K.EYTM#SWMK.E211 | +1 | 1291.5378 | 1291.5349 | Trypsin |
a The photolabeled peptide is shown between periods. # indicates that a 216.0762-Da modification was detected. Amino acid numbering is according to full-length rat SIRT2.
b Chymo indicates chymotrypsin.