FIGURE 1.
Generation and expression analysis of P3h2-null (P3h2n/n) mice. A, knock-out first allele mice (−, P3h2+/−) were generated using embryonic stem cells obtained from EUCOMM. Conditional wild-type mice (c, P3h2c/+) were generated by removing the lacZ expression cassette, using Rosa26-Flipase. Null mice (n, P3h2n/+) were generated by removing exon 3 using CMV-Cre. Mice were maintained on a C57/BL6J background. P3h2 expression was found throughout multiple tissues of the whole-mount mouse (B) but was localized to the glomeruli of kidney sections (C) as determined by β-galactosidase staining. D, immunoblot analysis using a polyclonal P3h2 antibody against total protein extracts from whole kidneys of wild-type (WT) and P3h2−/− (KO) mice supported the knock-out of protein expression in the P3h2n/n mice.