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. 2015 Feb 2;290(13):8613–8622. doi: 10.1074/jbc.M114.634915

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Tissue-specific hydroxylation patterns in type I collagen from bovine eye tissues. LC-MS profiles of in-gel trypsin digests of the α1(I) collagen chains from bovine sclera and cornea. A, MS profile of α1(I) from bovine cornea reveals no 3-hydroxylation at the (GPP)_n. B, MS profile of the α1-chain from bovine scleral type I collagen reveals a similar hydroxylation to that observed in mouse tendon and sclera. The trypsin digested peptide is shown with P^# indicating 3Hyp and P* indicating 4Hyp.