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. 2015 Feb 13;290(13):8632–8643. doi: 10.1074/jbc.M114.609677

FIGURE 1.

FIGURE 1.

Effects of CNL on MDA-MB-231 cells. A, MDA-MB-231 cells were treated with 1.56–50 μm ghost liposomes or CNL for 24 h under adherent conditions, followed by MTS assay for cell viability. The IC50 of CNL was 8.93 μm. n = 4. Bar, S.E. B, MDA-MB-231 cells were treated with 1.56–50 μm ghost liposomes or CNL for 24 h under nonadherent conditions, followed by MTS assay for cell viability. The IC50 of CNL was 11.0 μm. n = 3. Bars, S.E. C, MDA-MB-231 cells were treated with 5 μm ghost liposomes or CNL for 24 h, followed by Western blot analysis of CD44 and β-actin (loading control). D, siRNA results in 97% knockdown of CD44s in MDA-MB-231 cells. Cells were treated with 100 nm GAPDH siRNA or CD44 siRNA for 48 h, followed by Western blot analysis of CD44.