Fig. 7.

(A) Time montage of raw single image frames for a trapped S. pombe cell. (B) A cell was immobilised and imaged with transmitted white light. (C) A zoomed image where the septum is clearly visible, and the cell is nearing fission. (D) Epifluorescence image of the same cell showing the fluorophores clustered around the two separate centromeres. (E) Corresponding PALM image of the fluorophore positions. (F) Increased resolution image of the fluorophore centres showing the level of detail achieved.