Skip to main content
. 2015 Mar 24;108(6):1390–1399. doi: 10.1016/j.bpj.2015.02.010

Figure 1.

Figure 1

Conformational changes in the transport cycle and the crystal structure of inward-facing LeuT. (A) Schematic representation of the transport cycle divided into three conformational states with the substrate (purple) and the cotransported Na+ ions (yellow). (B) Comparison of the outward- and inward-open state of LeuT (PDB: 3TT1 and PDB: 3TT3 (24)). The five helices displaying the largest conformational change between the two states (TM1–2 and TM5–7) are shown as ribbons, with TM1 (red), TM2 (orange), TM5 (light green), TM6 (dark green), TM7 (turquoise), and TM3-4 and TM8-10 as gray cylinders. TMs representing the inward-open state (bright colors); TMs corresponding to the outward-open state (dark colors). (C) The structure of the inward-open LeuT is shown (gray) with TM1 (red), TM6 (dark green), TM7 (light green), and TM8 (cyan). The two chains in the Fab domain are shown (blue and violet). The four mutated residues (Tyr-268-Ala in TM6, Lys-288-Ala in TM7, and Thr-354-Val and Ser-355-Ala in TM8) are highlighted (as spheres) and colored by element (carbon atoms shown in orange). To see this figure in color, go online.