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Second round of nested PCR to detect Plasmodium falciparum, indicating positivity at the expected size (205 bp). Lane 1- 50-bp DNA Step Ladder (Promega), lane 2 - negative control, lane 3 - positive control, lanes 4-9 - samples, lane 10 - control reagent; lane 11 - Gel Pilot Wide Range Ladder (100) (Qiagen).
