Table 3.

Correction to WT and L529I α, β-parameters obtained after the fitting procedure for NS1643 effect using the different mechanisms depicted in Fig. 6

Transition description	Label	M1		M2		M3		M4
		a	b	a	b	a	b	a	b
WT + NS1643
C3D → C2D	aed	1.54	1.00	0.76	1.00	1.62	1.00	1.02	1.00
C2D → C3D	bed	0.36	1.00	0.055	1.00	0.22	1.00	0.15	1.00
C2D → C1D	aind	0.32	—	1.66	—	1.39	—	1.51	—
C1D → C2D	bind	0.083	—	1.13	—	1.09	—	1.00	—
C1D → OD	aad	2.30	0.59	1.32	0.39	1.32	0.61	1.36	0.59
OD → C1D	bbd	0.71	1.00	0.48	1.00	0.42	1.00	0.43	1.00
OD → ID	bid	0.62	1.00	0.61	1.00	0.61	1.00	0.61	1.00
ID → OD	aid	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
O + D → OD	kon_Oa	—		4.4 × 10−1		3.20 × 10−1		1.00 × 10−2
C + D → CD	kon_Ca	2.44		1.1 × 10−1		9.7010−1		1.00 × 10−2
OD ↔ O + D	Kd_Ob	—		3.07		3.11		3.05
CD ↔ C + D	Kd_Cb	9.46		7.06		12.6		6.25
F value		1.43 × 10−5		1.55 × 10−6		2.76 × 10−5		2.74 × 10−6
L529I + NS1643
C3D → C2D	aed	0.79	1.14	9.9	0.57	4.71	0.78	6.47	0.61
C2D → C3D	bed	0.42	4.13 × 10−8	1.49 × 10−2	1.10	6.73 × 10−3	0.96	1.27 × 10−5	1.50
C2D → C1D	aind	0.16	—	0.15	—	0.28	—	0.16	—
C1D → C2D	bind	0.45	—	0.69	—	0.76	—	1.06	—
C1D → OD	aad	1.00	0.71	0.56	0.56	0.48	0.65	1.83	0.52
OD → C1D	bbd	0.81	0.90	0.81	0.85	0.76	0.83	0.81	0.83
OD → ID	bid	0.60	1.00	0.56	1.00	0.55	1.00	0.56	1.00
ID → OD	aid	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
O + D → OD	kon_Oa	—		1.00 × 10−2		1.17 × 10−2		1.01 × 10−2
C + D → CD	kon_Ca	1.00 × 10−2		1.00 × 10−2		1.06 × 10−2		1.00 × 10−2
OD ↔ O + D	Kd_Ob	—		9.76		10.12		9.94
CD ↔ C + D	Kd_Cb	2.53		1.34		0.075		2.21
F value		4.10 × 10−4		2.57 × 10−4		2.41 × 10−4		1.28 × 10−4

Experimental data obtained from hEK cells at room temperature. Underlined numbers are fixed values. K_d_C and k_on_C are assumed to be the same for all closed states.

^aUnits in μM⁻¹ ms⁻¹.

^bUnits in μM.