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. 2015 Mar 24;108(6):1480–1483. doi: 10.1016/j.bpj.2015.02.016

Figure 1.

Figure 1

Examples of cargo dynamics at MT intersections in vivo and in vitro. (A) A melanosome moving along a MT in a melanophore in vivo encounters another MT that crosses its movement path. Left panel: low-magnification fluorescence image of the melanophore periphery. Right panel, top row: successive high-magnification images of the boxed images are shown in the left panel; bottom row: schematic representation of the position of the melanosome (red circle) and MTs (green lines) in the images shown in the top row. Numbers indicate time in seconds; magnification bars are 10 μm (left panel) and 5 μm (right panel). A collision of the melanosome with the crossing MT blocks its movement and leads to deformation of both crossing MTs. (B and C) Examples of cargo dynamics at MT crossings in vitro: an MT deformation event (B) and a very long dwell event (C). Numbers indicate time in seconds. Top rows: still frames of beads and MTs. Bottom rows: schematic representations of the positions of beads (red) and MTs (green) in the images shown in the top row (frame timing indicated). Blue lines show deformed geometries; fields of view: 8.5 × 4.3 μm (B) and 6.0 × 9.4 μm (C).