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. Author manuscript; available in PMC: 2015 Sep 25.
Published in final edited form as: Nat Commun. 2015 Mar 25;6:6667. doi: 10.1038/ncomms7667

Figure 2.

Figure 2

Pharyngeal GRNs respond to sweet compounds (a) Immunofluorescence of anti-GFP (green) and nc82 (magenta) in the SEZ of flies expressing GCaMP3 under control of Gr43a-GAL4. Dotted line shows area imaged in panel (b). (b) Single optical section of baseline GCaMP3 fluorescence in pharyngeal GRN axon terminals. Scale bars are 20 μm in a-b. (c) Heat map showing change in GCaMP3 fluorescence during ingestion of 1 M fructose. (d) Representative trace of fluorescence change of GCaMP3 in Gr43a axon terminals during ingestion of 1 M fructose. Arrow indicates time at which stimulus is applied to the proboscis to initiate feeding. (e) Peak fluorescence changes of GCaMP3 in Gr43a axon terminals during ingestion of 1 M solutions of the indicated compounds. Values represent mean +/− s.e.m. for n = 5 flies per stimulus (n = 4 for sorbitol), with data collected over at least 2 days. Asterisks indicate significant difference from water by one-way ANOVA with Bonferroni correction for multiple comparisons: **p < 0.01, ***p < 0.001, ns = not significant.