Figure 1.

Validating the pB-Tet-GOI System for Transgene Manipulation

(A) Schematic of pB-Tet-GOI system for transposon-mediated integration along with inducible and reversible transgene expression.

(B–D) Response plasmids utilized for directed differentiation experiments.

(E) Western blot analysis of response groups grown with and without dox.

(F–K) Immunocytochemical staining for HA (DLX2 epitope tag), GFP (dox reporter), TagBFP2 (constitutive reporter), and NGN2 in HuNPCs nucleofected with indicated response plasmids.

Scale bar, 100 μm.

(L and M) Quantification of HuNPCs harboring indicated response plasmids and differentiated for 4 days (I) or 2 weeks (J) after dox.

Error bars represent mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01; n = 3 biological replicates per condition per time point.

See also Figure S1.