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. 2013 Oct 1;1(1):4. doi: 10.1186/2050-490X-1-4

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© Coletti et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kinetics of skeletal muscle regeneration following focal injury in mouse. Hematoxilin and eosin staining (H&E) of murine Tibialis anterior muscle (only a portion of the muscle is shown) subjected to freezing by applying a liquid nitrogen-cooled steel forceps to the surface (facing right in the picture) of the muscle for 10 seconds. The muscle was collected and analyzed 3, 6, 8 and 10 days following injury (from left to right). The inset shows a higher magnification image of regenerating fibers 8 days after injury: hallmarks of regenerating fibers include reduced fiber size and centrally located nuclei.