Figure 6.

SSA Induces Molecular Changes to the Niche and Its Ability to Support Hematopoiesis

Transcriptome analysis was performed on purified sinusoidal CD45⁻TER119⁻ cells from 2- and 9-month untreated mice, 9-month shSSA control, and 9-month SSA mice at days 2, 4, 7, and 10 after surgery (n = 1, ten mice pooled/sample).

(A) Pattern discovery (correlation >0.7) identified 132 probes that demonstrated an increasing gene expression trend toward young profiles following castration of middle-aged mice.

(B) qPCR of Foxo1 expression in pre-OBLs purified from untreated 2- or 9-month mice, and 9-month d2shSSA or 9-month d2SSA mice.

(C–E) Expression of Cxcl12, Kitlg, Spp1, Ang1, Jag1, Vcam1, Bmp4, Il7, Tgfb, and Igf1 in endothelial cells (C), pre-OBLs (D), and OBLs (E) from 9-month d2SSA and 9-month d7SSA. Expression is represented as 9-month d7SSA relative to 9-month d7shSSA control mice, n = 3–11 independent experiments.

^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See also Figure S4.