Figure 7.

SSA Promotes the Ability of the Niche to Support Hematopoiesis

Lethally irradiated 9-month d7shSSA or 9-month d7SSA were reconstituted with either 5 × 10⁵ or 5 × 10⁶ BM cells from untreated 2-month CD45.1 donors (n = 5–6/group).

(A) Concatenated flow cytometry plots showing the proportion of donor LSK cells at 28 days after transplant.

(B) Absolute number of donor-derived LSK cells in the BM 28 days after transplant.

(C) Concatenated flow cytometry plots showing total donor reconstitution in the spleen at days 28 and 84 after transplant.

(D) Absolute number of donor-derived cells in the spleen at days 28 and 84 after transplant.

(E) Absolute number of donor-derived Gr1⁺CD11b⁺ granulocyte, Gr1^loCD11b⁺ monocyte/macrophage, B220⁺ B cells, and TCRβ⁺ T cells in the spleen 84 days after transplant.

Results are expressed as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01.