Table 1.
Summary of the In Vivo Engraftment Experiments
| Population Tested | Cytokines in FLK1 Culture | n | Engrafted Mice | Chimerism Frequency at 4 Weeks (Percentage CD45.2+) |
|---|---|---|---|---|
| FLK1+ | no further culture | 2 | 0/8 | 0 |
| cKIT+ day 1 | BAFV | 6 | 14/26 | 4, 5.9, 6.5, 0.37, 4.5, 2.32, 0.77, 21, 3.36, 2.17, 10.04, 15.66, 5.04, 16.36 |
| BAV | 2 | 3/8 | 0.4, 19.9, 15.65 | |
| AV | 1 | 1/4 | 0.43 | |
| BNVF | 1 | 0/4 | 0 | |
| BNV | 1 | 1/4 | 0.09 | |
| BFV | 1 | 0/4 | 0 | |
| cKIT+ day 2 | BAV(day 1)-BV(day 2) | 7 | 9/28 | 2.96, 15.09, 0.76, 0.91, 1.44, 0.18, 0.58, 0.44, 0.26 |
| BAV(day 1)-A(day 2) | 1 | 2/4 | 0.12, 2.9 | |
| BAV(day 1)-SB(day 2) | 1 | 2/4 | 0.19, 2.5 | |
| BAV(day 1)-B(day 2) | 1 | 1/4 | 1.5 | |
| BAV(day 1)-none(day 2) | 1 | 1/3 | 0.24 | |
| BAFV | 2 | 5/12 | 0.78, 1.04, 1.3, 2.9, 6.1 | |
| BAV | 1 | 1/8 | 1.42 | |
| BNVF | 1 | 0/4 | 0 | |
| BNV | 1 | 0/4 | 0 | |
| cKIT+ day 3 | BAV(day 1)-BV(day 2) | 2 | 0/12 | 0 |
B, BMP4; A, Activin A; V, VEGF; F, FGF2; N, Nodal; SB, SB-431542. See also Figure S5.