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. 2015 Feb 26;4(3):459–472. doi: 10.1016/j.stemcr.2015.01.017

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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MSCs Expanded in the Presence of WNT3A and FGF2 Display Accelerated Chondrogenesis

(A and B) Relative gene expression levels of COL2 and COL2B in pellet cultures from cells expanded in the indicated conditions after 3 (A) or 5 (B) weeks of chondrogenic induction. Values represent means ± SEM (n = 3 donors with three pellets per donor). ^∗∗p < 0.01, ^∗∗∗p < 0.001.

(C) Relative glycosaminoglycan content of pellet cultures from cells expanded in the indicated conditions. Lines indicate the polynomial regression representatives of the glycosaminoglycan accumulation in the WF-MSCs (+WNT+FGF; solid) and F-MSCs (+FGF; dotted).