Table 1.
List of Metrics of Sarcomere Organization Developed, Integrated, or Updated for This Study
| Metric Number | Metric Name | Metric Description |
|---|---|---|
| 1 | sarcomere length (SL) | The average distance between Z-disks in the entire field of view. |
| 2 | total energy | The total amount of spatially varying immunosignal. |
| 3 | sarcomeric energy | The amount of immunosignal with a spatial periodicity given by SL. |
| 4 | sarcomeric packing density (SPD) | The fraction of immunosignal that localized in a regular lattice at a distance SL. |
| 5 | orientational order parameter (OOP) | The degree of alignment of all foreground elements in the field of view. |
| 6 | sarcomeric OOP (OOP1) | The degree of alignment of foreground elements that are oriented orthogonally to the actin bundles (Z-disks). |
| 7 | nonsarcomeric OOP (OOP2) | The degree of alignment of foreground elements that are oriented parallel to the actin bundles (Z-bodies). |
| 8 | Z-disks relative presence (γ) | The fraction of foreground elements that are recognized as Z-bodies. |
| 9 | weighted OOP | Calculated by multiplying the sarcomeric OOP (OOP1) and the weight γ. In this sense, it represents both the abundance and relative alignment of the Z-disks in the imag. |
| 10 | coverage quality control | Calculated as the percentage of the image pixels that have an intensity value higher than user-specified threshold. It estimates the α-actinin coverage. |
| 11 | coherency quality control | Calculated as the percentage of α-actinin-positive pixels that have a value of the coherency higher than a user-selected threshold. It is useful for artifact removal and for image quality control. |
See Figure S1 for a schematic representation of the role of each parameter.