Table 2. Mean RT-qPCR threshold (Ct) values of 12 references genes in juvenile (13-17 days post natal) rat tissues.

Mean ± standard deviation (SD) was calculated from 4 biological replicates (n = 4).

Gene	Liver (Mean ± SD)	Fat pad (Mean ± SD)	Adrenal (Mean ± SD)	Prostate (Mean ± SD)	Testis (Mean ± SD)	Ovary (Mean ± SD)	All (Mean ± SD)
Actb	23.0 ± 0.23	22.0 ± 0.31	20.5 ± 0.31	20.0 ± 0.30	19.7 ± 0.69	20.4 ± 0.32	20.9 ± 1.25
B2m	23.6 ± 0.21	22.7 ± 0.19	22.1 ± 0.32	23.9 ± 0.26	23.9 ± 0.60	22.6 ± 0.51	23.2 ± 0.80
Gapdh	24.1 ± 0.19	24.2 ± 0.74	22.7 ± 0.52	23.9 ± 0.17	23.2 ± 0.32	23.2 ± 0.18	23.6 ± 0.69
Hprt	28.0 ± 0.37	27.1 ± 0.16	26.2 ± 0.35	26.7 ± 0.24	25.7 ± 0.26	26.0 ± 0.26	26.6 ± 0.81
Pgk1	27.9 ± 0.32	26.4 ± 0.57	24.7 ± 0.35	27.3 ± 0.39	27.3 ± 0.09	25.5 ± 0.21	26.5 ± 1.18
Rn18s	13.8 ± 0.45	12.5 ± 0.19	13.9 ± 0.33	14.2 ± 0.27	13.9 ± 0.65	14.3 ± 0.38	13.8 ± 0.69
Rpl13a	25.4 ± 0.17	23.8 ± 0.12	23.2 ± 0.18	23.1 ± 0.26	22.9 ± 0.06	22.3 ± 0.06	23.5 ± 1.02
Rps18	22.3 ± 0.33	21.4 ± 0.18	20.8 ± 0.11	20.5 ± 0.33	20.5 ± 0.24	19.6 ± 0.22	20.9 ± 0.88
Rps29	21.6 ± 0.24	21.5 ± 0.12	21.9 ± 0.12	21.3 ± 0.18	21.2 ± 0.76	19.3 ± 0.11	21.1 ± 0.91
Sdha	26.5 ± 0.26	25.6 ± 0.19	24.3 ± 0.36	26.0 ± 0.21	25.1 ± 0.11	25.1 ± 0.38	25.4 ± 0.76
Tbp	32.7 ± 0.31	29.8 ± 0.23	30.2 ± 0.37	30.1 ± 0.31	28.2 ± 0.23	28.9 ± 0.13	30.0 ± 1.43
Ubc	23.9 ± 0.26	23.0 ± 0.26	22.3 ± 0.29	21.7 ± 0.16	22.0 ± 0.18	22.0 ± 0.14	22.5 ± 0.78