Table 3. Mean RT-qPCR threshold (Ct) values of 12 reference genes in adult rat tissues.

Mean ± standard deviation (SD) was calculated from 4 biological replicates (n = 4).

Gene	Liver (Mean ± SD)	Fat pad (Mean ± SD)	Prostate (Mean ± SD)	Testis (Mean ± SD)	All (Mean ± SD)
Actb	23.1 ± 0.16	21.3 ± 0.16	21.1 ± 0.49	21.0 ± 0.30	21.6 ± 0.92
B2m	21.4 ± 0.38	21.1 ± 0.27	23.9 ± 0.81	23.3 ± 0.26	22.4 ± 1.33
Gapdh	20.7 ± 0.12	23.0 ± 0.25	24.6 ± 0.25	23.7 ± 0.31	23.0 ± 1.48
Hprt	27.7 ± 0.39	27.1 ± 0.11	26.8 ± 0.26	27.0 ± 0.17	27.1 ± 0.43
Pgk1	25.8 ± 0.35	25.2 ± 0.18	26.9 ± 0.47	26.9 ± 0.32	26.2 ± 0.81
Rn18s	14.8 ± 1.55	12.7 ± 0.28	14.7 ± 0.94	14.4 ± 1.56	14.1 ± 1.38
Rpl13a	25.6 ± 0.35	24.3 ± 0.40	23.6 ± 0.16	24.5 ± 0.23	24.5 ± 0.79
Rps18	23.1 ± 0.82	22.3 ± 0.75	21.0 ± 0.27	19.8 ± 0.21	21.5 ± 1.38
Rps29	23.2 ± 0.25	22.0 ± 0.38	21.1 ± 0.68	21.5 ± 0.29	22.0 ± 0.90
Sdha	25.0 ± 0.42	24.7 ± 0.19	26.4 ± 0.22	25.2 ± 0.20	25.3 ± 0.69
Tbp	31.6 ± 0.61	30.2 ± 0.25	30.3 ± 0.25	24.8 ± 0.40	29.2 ± 2.73
Ubc	22.1 ± 0.42	21.8 ± 0.17	21.5 ± 0.16	20.1 ± 0.30	21.4 ± 0.84