Table 5. Mean RT-qPCR threshold (Ct) values of 12 reference genes in adult tissues from rats exposed to hPFNA or hPFNA/Mix.

Mean ± standard deviation (SD) was calculated from 4 biological replicates (n = 4.)

Gene	Liver + hPFNA (Mean ± SD)	Liver + hPFNA/mix (Mean ± SD)	Fat pad + hPFNA (Mean ± SD)	Fat pad + hPFNA/mix (Mean ± SD)	Testis + hPFNA (Mean ± SD)	Testis + hPFNA/mix (Mean ± SD)
Actb	22.8 ± 0.06	22.5 ± 0.37	21.6 ± 0.30	21.6 ± 0.34	20.3 ± 0.25	20.7 ± 0.29
B2m	21.4 ± 0.12	21.5 ± 0.27	20.8 ± 0.15	21.2 ± 0.53	23.6 ± 0.47	23.8 ± 0.44
Gapdh	19.8 ± 0.18	19.7 ± 0.16	24.1 ± 0.53	23.5 ± 0.72	23.9 ± 0.30	22.2 ± 1.34
Hprt	26.5 ± 0.27	26.4 ± 0.17	26.9 ± 0.18	26.7 ± 0.81	26.9 ± 0.30	27.2 ± 0.24
Pgk1	24.3 ± 0.24	24.1 ± 0.26	25.6 ± 0.34	25.4 ± 0.33	27.3 ± 0.45	27.8 ± 0.42
Rn18s	15.1 ± 1.27	14.1 ± 0.17	12.6 ± 0.27	13.4 ± 1.10	12.2 ± 0.04	14.1 ± 0.89
Rpl13a	23.1 ± 0.19	23.0 ± 0.33	23.8 ± 0.29	23.8 ± 0.31	24.4 ± 0.33	24.5 ± 0.21
Rps18	20.2 ± 0.21	20.0 ± 0.37	21.4 ± 0.33	21.5 ± 0.25	19.7 ± 0.26	19.9 ± 0.25
Rps29	21.6 ± 0.18	21.5 ± 0.28	21.8 ± 0.32	21.8 ± 0.23	21.3 ± 0.28	21.5 ± 0.22
Sdha	24.3 ± 0.28	24.1 ± 0.21	24.8 ± 0.33	24.6 ± 0.53	24.9 ± 0.25	25.0 ± 0.31
Tbp	30.5 ± 0.41	30.2 ± 0.23	29.8 ± 0.14	29.9 ± 0.09	24.3 ± 0.33	24.5 ± 0.33
Ubc	21.0 ± 0.21	20.8 ± 0.24	21.8 ± 0.08	21.4 ± 0.54	19.8 ± 0.39	20.0 ± 0.51