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. 2015 Mar 27;6:206. doi: 10.3389/fpls.2015.00206

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Copyright © 2015 Hong, Chu, Zhang, Ghosh, Gong and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lugol’s staining showing accumulation patterns of starch granules in the columella root cap of 4-day-old WT and mutant genotypes. (A) WT (n = 14). (B) wox5-1 (n = 15). (C) fez-2 (n = 11). (D) 35S::miR160 (n = 15). (E) WT treated with 5 nM oryzalin for 24 h (n = 11). (F) atr (n = 12). (G) atm-1 (n = 12) and (H) wee1 (n = 12). (I) Quantification of the number of layers of unstained columella root cap cells. Error bars represent standard error of the mean. **: P < 0.01, t-test. **** represents position of the QC. CSC: layer of columella root cap stem cells; CSCD: layer of differentiating columella root cap stem cell daughters; 1st DCC: 1st layer of fully differentiated columella root cap cells; 2nd DCC: 2nd layer of fully differentiated columella root cap cells; 3rd DCC: 3rd layer of fully differentiated columella root cap cells. Scale bars: 20 μm.