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. 2015 Mar 27;6:206. doi: 10.3389/fpls.2015.00206

FIGURE 2.

FIGURE 2

EdU Staining patterns in the columella root cap of 4-day-old WT and mutant genotypes. (A) WT (n = 18). (B) wox5-1 (n = 11). (C) fez-2 (n = 15). (D) 35S::miR160 (n = 10). (E) WT treated with 5 nM oryzalin (n = 16) for 24 h. (F) atr (n = 12). (G) atm-1 (n = 12) and (H) wee1 (n = 12). All WT and mutant seedlings were treated with 10 μM EdU for 24 h before imaging. (I) Quantification of the percentage of roots with EdU stain in the indicated columella root cap layer. **** represents position of the QC. C1: 1st columella root cap layer below the QC, corresponding to the layer of columella root cap stem cells (CSC); C2: 2nd columella root cap layer below the QC, corresponding to the layer of differentiating columella root cap stem cell daughters (CSCD); C3 to C5: 3rd to 5th columella root cap layers below the QC, corresponding to 1st to 3rd layers of fully differentiated columella root cap cells (1st to 3rd DCCs). C5+: extra columella root cap layer(s) in 35S::miR160. Note that cell fate in the columella root cap may be altered in the mutant genotypes. Scale bars: 20 μm.