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. 2015 Mar 27;6:194. doi: 10.3389/fpls.2015.00194

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Copyright © 2015 Falter, Ellinger, vonHülsen, Heim and Voigt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dissection and catapulting of 30 μm-disks from ACT-prepared adaxial leaf epidermis. Application of LM coupled with laser pressure catapulting (LMPC) on ACT-prepared A. thaliana adaxial leaf epidermis. Epidermis (A) before and (B) after microdissection of a 30 μm disk; (C) removal of 30 μm-disk from epidermal tissue by LMPC. (D) ACT-prepared adaxial leaf epidermis 3 days post-inoculation with the powdery mildew Golovinomyces cichoracearum, additionally stained with aniline blue to visualize callose deposition. Red arrow indicates 30 μm-disk including pathogen-induced, callosic papillae (spots with light-blue fluorescence). (E,F) Bright-field micrographs of the same epidermis area as in (D,E) after microdissection and (F) after LMPC of a 30 μm-disk. PALM MicroBeam LM system used for LMPC and micrograph acquisition. Scale bars = 50 μm.