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. 2015 Mar 27;9:78. doi: 10.3389/fnbeh.2015.00078

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Copyright © 2015 Manto, Honnorat, Hampe, Guerra-Narbona, López-Ramos, Delgado-García, Saitow, Suzuki, Yanagawa, Mizusawa and Mitoma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of GAD65Ab on association of GAD65 with GABAergic vesicles. Synaptic vesicles were incubated with monoclonal antibodies b78, HAA1, b96.11, or N-GAD65mAb for 1 h on ice. No antibody was added for the control sample. GABAergic vesicles were isolated as outlined in the inset sketch and the immunoprecipitated proteins were analyzed for the presence of synaptophysin by Western blot. (A) Representative Western blot analysis. (B) Densiometric analysis from three independent experiments. Results are presented as percentage of the corresponding control sample. Error bars represent the standard deviation of the mean. Inset: schematic representation of the experimental set-up.