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. 2015 Mar 27;9:40. doi: 10.3389/fnana.2015.00040

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Copyright © 2015 Wang, He, Zhao, Feng, Lin, Sun, Ding, Xu, Luo and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of direct inputs to POMC and AgRP neurons in the ARC using stereotaxic injection of the modified rabies virus into Cre-transgenic mice. (A) Design of recombinant AAV-DIO-EGFP-TVA, AAV-DIO-RG, and modified rabies virus. (B) Experimental design. (C) Selective expression of EGFP-TVA in POMC-expressing neurons following the unilateral injection of AAV-DIO-EGFP-TVA into the ARC of a POMC-Cre mouse. (D), EGFP-TVA expression pattern in the ARC of an AgRP-Cre mouse. (E,F) Characterization of the distribution pattern of starter neurons (green) and retrogradely labeled neurons (red) in the ARC of POMC-Cre (E) and AgRP-Cre (F) mice. (G) Transsynaptic labeling (red) was observed in a Cre-transgenic mouse but not a wildtype control mouse. Blue indicates cell nuclear staining with DAPI.