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. 2015 Mar 27;9:40. doi: 10.3389/fnana.2015.00040

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Copyright © 2015 Wang, He, Zhao, Feng, Lin, Sun, Ding, Xu, Luo and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of whole-brain efferents of POMC neurons. (A) Design of recombinant AAV strains for Cre-dependent cell labeling. (B) AAV-DIO-mtdTomato and AAV-DIO-EmGFP were injected into the ARC and NTS of POMC-Cre mice respectively. (C) Direct comparison of the axon projections of the ARC and NTS POMC neurons. The dominant projections of the ARC (red, indicated with red words) and NTS (green, indicated with white words) were represented in a series of sagittal sections. DTg, dorsal tegmental nucleus; Rt, reticular thalamic nucleus. (D) Representative sagittal sections show the axon projections of AgRP neurons. LDTg, laterodorsal tegmental nucleus.