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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nano Life. 2014 Jun 1;4(2):1450004. doi: 10.1142/s1793984414500044

Fig. 4.

Fig. 4

Dot-blot assay of ELP + M2e protein. Dot-blot assay was performed to study antigen recognition on (a) ELP(A2YA2)24 + M2e and (b) ELP(A2YA2)36+ M2e. Different concentrations of ELP + M2e fusion proteins, free M2e peptide and ELP without M2e were spotted. The concentrations for each spot are mentioned at the top. Color development revealing M2e recognition on ELP + M2e fusion protein can be seen in the top-most rows for both (a) and (b). No color development was observed for the middle and bottom rows where free M2e peptide and ELP without M2e were spotted for both (a) and (b), demonstrating that M2e in ELP + M2e is specifically recognized by anti-M2e antibodies, which do not cross react with ELP (color online).