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Reactivity of mouse serum-IgG (1:20 dilution) against native M2 expressed on influenza A virus WSN-H1N1. Groups of mice (n = 5 per group) were vaccinated on days 0 and 21. Mouse serum from day 42 was diluted to 1:20 and assessed using ELISA to determine IgG against WSN-H1N1. OD measured at 492 nm wavelength. mean ± SEM.
