Figure 1.
L1TD1-Interacting Proteins Are Involved in RNA Processing
(A) Schematic diagram of the human L1TD1 protein structure: coiled coil (CC) domain, RNA recognition motif (RRM) domain, and carboxyl tail domain (CTD). Regions targeted by antibodies 1 and 2 used in the IPs are shown. Protein domain borders are based on Khazina et al. (2011).
(B) Venn diagram of the proteins identified in the L1TD1 interactome (shaded area) based on MS analysis of three biological replicates.
(C) Clusters of the most enriched biological processes in the L1TD1 interactome. Data from the STRING interaction database (Szklarczyk et al., 2011) were visualized using Cytoscape 2.8.1 (Shannon et al., 2003).
(D) The three most significant functions of L1TD1 interactome proteins based on Ingenuity Pathway Analysis (IPA). Proteins validated experimentally are shown in red.
(E) Western blot (WB) validations of individual protein-protein interactions in L1TD1, IgG control, and unbound (UB) IP reactions. NC, negative control. hESC line HS360. CTNNB1 separate IP reaction, DDX3X and PABPC1 separate IP reaction, hESC line H9.
(F) Protein expression of the interacting proteins after L1TD1 silencing. NT, non-targeting. hESC line HS360. Quantifications can be found in the Supplemental Results.