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. 2014 Oct 31;6(3):e954436. doi: 10.4161/19382014.2014.954436

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© 2014 The Author(s). Published by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Immunofluoresence for NEUROG3 in human fetal pancreas. (A–E) Immunofluorescence for NEUROG3 at 14 wpc. (A) Arrowheads point to green PCNA-positive cells while arrows point to separate red NEUROG3-positive cells. (B) and (C) Arrows point to hormone-negative NEUROG3-positive cells. Arrowhead points to a very rare NEUROG3-positive cell with faint somatostatin (SS) staining. (D) and (E) Arrowheads point to NEUROG3-positive nuclei visible in (D) but not (E) following pre-incubation with full-length human NEUROG3 protein. Amplification of the red gain with DAPI counterstaining to investigate the loss of nuclear staining has introduced some background cytoplasmic staining. Scale bar represents 50 μm.