Figure 4.

Formation of Hex in aqueous dispersions of SPH (A) and SPC (B) under the action of MPO/H₂O₂/Cl⁻ system. 1—sphingolipid dispersion; 2—sphingolipid dispersion/0.3 mM H₂O₂; 3—sphingolipid dispersion/MPO; 4—sphingolipid dispersion/MPO/0.3 mM H₂O₂; 5—sphingolipid dispersion/MPO/0.6 mM H₂O₂; 6—sphingolipid dispersion/MPO/1.0 mM H₂O₂; 7—sphingolipid dispersion/MPO/1.2 mM H₂O₂. The reactions were conducted in 50 mM PBS, 140 mM NaCl, pH 4, T = 37°C, t = 60 minutes. The sphingolipid concentration in the system thus obtained was 5 mM, and that of MPO was 1.5 U/mL. Error bars indicate SD of the means (n ≥ 3).