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. 2015 Mar 23;10:2313–2323. doi: 10.2147/IJN.S75011

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© 2015 Meng et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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The transcription markers of primary hepatocytes on the hybrid matrix with different concentrations of FBS on days 1 and 5.

Notes: The expression of hepatic markers at the gene level was analyzed by using RT-PCR (A). The relative expression levels of the markers ALB (B), HNF-4α (C), and ASGPR (D) were quantified by using ImageJ software and normalized to the GAPDH housekeeping gene. The primary hepatocytes cultured on gelatin with 10% FBS are used as a control. The expression level of each maker on gelatin after 1 day culture is considered as 100%. The hybrid matrix was made up of EFC and PVLA. The symbols * and **denote statistical significance at P<0.05 and at P<0.01.

Abbreviations: RT-PCR, reverse transcription polymerase chain reaction; G, gelatin; FBS, fetal bovine serum; ALB, albumin; HNF-4α, hepatocyte nuclear factor-4 alpha; ASGPR, asialoglycoprotein receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; EFC, E-cadherin-Fc; PVLA, poly-(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide).