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. Author manuscript; available in PMC: 2015 Mar 27.
Published in final edited form as: Methods Mol Biol. 2014;1093:137–148. doi: 10.1007/978-1-62703-694-8_11

Fig 3. piRNP purification by immunoprecipitation with the Y12 antibody.

Fig 3

Y12 immunoprecipitate from BmN4 cells, and anti-Flag immunoprecipitates from BmN4 cells expressing Flag-SIWI or Flag-BmAGO3 were subjected to RNA extraction, 5’-end labeling of the extracted RNA, and denaturing PAGE. piRNAs were clearly observed in Y12 immunoprecipitate as well as PIWI’s, which indicated the successful purification of piRNPs with the Y12 antibody.