FIG. 3.

Suboptimal concentrations of Rosu+Bex decreases Ang-II-induced chemokine production in HUAEC. Cells were treated with Rosu (10 nM), Bex (0.3 μM), or a combination of Rosu (10 nM) plus Bex (0.3 μM) for 20 h and then stimulated with Ang-II (1 μM) for 4 h. GROα (A), IL-8 (B), MCP-1(C), and RANTES (D) release was determined by enzyme-linked immunoassay (ELISA) in the cell-free supernatant. Results are expressed as pM concentration and presented as mean±SEM of n=7–9 independent experiments; **p<0.01 relative to the vehicle group, +p<0.05 or ++p<0.01 relative to the Ang-II stimulated cells. In a second set of experiments, HUAECs were treated with Rosu (10 nM), Bex (0.3 μM), or a combination of Rosu (10 nM) plus Bex (0.3 μM) for 20 h and then stimulated with Ang-II (1 μM) for 24 h. CX₃CL1 expression in HUAEC was determined by flow cytometry (E) and visualized by immunofluorescence (green). Nuclei were counterstained with DAPI; images are representatives of n=5–7 independent experiments per group (F). Results are the mean±SEM of n=5–7 independent experiments; *p<0.05 or **p<0.01 relative to the vehicle group, +p<0.05 relative to the Ang-II-stimulated cells.