Effects of blocking β-catenin activity by DKK and DNLEF1 in developing chicken liver. (A) Livers from controls, RCAS-DNLEF1, and RCAS-DKK. The diagram on the right shows the comparison of weights (n = 5). (B) Effects of blocking β-catenin on cell morphology, proliferation, and differentiation. Sections from control (left), RCAS-DNLEF1 (middle), and RCAS-DKK (right) were analyzed with H&E and stained with antibodies against PCNA, β-catenin, and L-CAM. Presence of glycogen and TUNEL were also analyzed. To verify viral transduction, the same areas analyzed for RCAS-DNLEF1 and RCAS-DKK were also stained with antibody 3C2 to viral protein, and antibodies to HA tage on RCAS-DKK.