Table 1. PCR primers used in current study.
Gene | Sequence | Amplicon Length | Cycles |
---|---|---|---|
| |||
VEGF-A | 5′ –ACCATGCCAAGTGGTCCCAGGC– 3′ (sense) | 250 | 35 |
5′ –TCCAGACCTTCGTCGTTGCAGC– 3′ (antisense) | |||
| |||
PDGF | 5′ –AGGGCTGTTCCGACAAGCGC– 3′ (sense) | 259 | 35 |
5′ –TGGCAGGTCCTCTCGGGAGC– 3′ (antisense) | |||
| |||
FGF-1 | 5′ –GCCATGGCTGAAGGCGAAATCACA– 3′ (sense) | 250 | 35 |
5′ –CACCTCCCCCACGCTTTCCG– 3′ (antisense) | |||
| |||
MMP-2 | 5′ –GATGTCGCCCCCAAAACGGAC– 3′ (sense) | 852 | 35 |
5′ –GTAGTCCTCGGTGGTGCCGC– 3′(antisense) | |||
| |||
TIMP-1 | 5′ –CCTGGCCTCTGGCATCCTGC– 3′ (sense) | 162 | 35 |
5′ –CGCTGGTACGAGGCGGTCTG– 3′(antisense) |