FIG 3. Assessment of the babA gene at early time points.
For strains that lacked the babA gene at 5 years post inoculation as determine by CGH, PCR amplification of babA was performed using primers babA-M3F and babA-M1R on 18 and 24 month post inoculation isolates. Individual isolates (numbered 1–7) from EH1 are shown here as a representative example. Seven individual isolates from 18 months post-infection and seven individual isolates from 24 months post-infection were examined. Individual strains are indicated by a “- “ followed by the isolate number. At 18 months, EH1 output strains are similar to the USU101 input strain; however, at 24 months, a doublet appears.