Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Int J Med Microbiol. 2015 Mar 6;305(3):392–403. doi: 10.1016/j.ijmm.2015.03.002

FIG 4. Detection of BabA by Western blot.

FIG 4

Protein was purified from single colony isolates from H and EH monkeys as early as 1 week to 120 months (10 years) post inoculation. Equal amounts of protein from lysates were run on a denaturing gel, and BabA expression was detected using poly-clonal rabbit antibodies. J99 and USU101 were included as positive controls, and a ΔbabA J99 strain was included as a negative control. Relative sizes as determined using a protein ladder are indicated; J99 BabA is known to run at approximately 75kD. BabB expression was also analyzed as a relative loading control. Lanes are labeled with the monkey designation followed by the time point. W, M, and Y stand for weeks, months, and years post inoculation, respectively.